Our current efforts are focused on designing new inhibitors against NDM-1 and CTX-M variants. Many synthetic molecules after being screened by QSAR and structure-based virtual screening approaches from different Databases are being tested against multidrug resistant clinical strains. Different Machine learning approaches are used to design lead molecules against enzymes. Moreover, these enzymes and their mutants are being cloned from clinical multi-drug-resistant strains to express proteins/enzyme which is being studied for their interaction and hydrolysis with inhibitors and drugs respectively in vitro using various microbiological and spectroscopic approaches. Moreover, inhibitor-enzyme sites are also being mapped through Crystallography to further understand the insight of binding interactions.